[Abstract] Chinangwa: Kudzidza maitiro eAgkistrodon acutus venom tumor suppressor component I (AAVC-I) pakuwedzera kwekudzivirira uye apoptosis yekenza yemapapu evanhu A549 masero.Nzira: Maitiro ekudzivisa maitiro eAAVC-I pane zvakasiyana-siyana pamasero eA549 e24h uye 48h akayerwa neMTT nzira;HE staining uye Hoechst 33258 fluorescence staining yakashandiswa kuona apoptosis kubva morphology;Kutaura kweprotein bax kwakaonekwa ne immunohistochemistry.Migumisiro: MTT yakaratidza kuti AAVC-Ini inogona kudzivisa kupararira kwemasero eA549 mune imwe nguva-inotenderera uye inotenderera-dose;Mushure mokunge AAVCI kurapwa kwemaawa 24, nuclear pyknosis, nuclear hyperchromatic uye apoptotic miviri yakaonekwa pasi pe microscope;Immunohistochemistry yakaratidza kuti avhareji optical density yakawedzera nekuwedzera kweiyo zvinodhaka, zvichiratidza kuti kutaura kweprotein yebax kwaienderana kumusoro-kwakarongedzwa.Mhedziso: Antitumor component I yeAgkistrodon acutus venom inogona kuvharidzira kenza yemapapu emunhu A549 masero uye kukurudzira apoptosis, iyo inogona kunge ine hukama nepamusoro-mutemo we bax kutaura.
Nguva yekutumira: Jan-16-2023