[Abstract] [Chinangwa] Kugadzirira IgY antibody kupesana neyakajairwa motif yemetalloproteinases kubva kuAgkistrodon acutus venom, uye kudzidza maitiro ayo pakuita kwehuturu hwenyoka [Nzira] Nekuongorora kutevedzana kwakajairwa kweakasiyana metalloproteinases kubva kuAgkistrodon acutus venom, isu takagadzira. uye yakagadzira iyo yakanyanya kuchengetedzwa antigen peptides PT1 uye PT2, iyo yakanyatsoenderana nebasa re enzyme;Huku dzakabaiwa ne conjugated compounds KLH-PT1 uye KLH-PT2 kuwana maIgY antibodies.Mutsara weIgY uye maitiro e-cross-reaction neAgkistrodon acutus uye Agkistrodon acutus venom zvakadzidzwa neELISA uye Western blot;Pakupedzisira, basa reIgY rakaongororwa ne-anti-subcutaneous hemorrhage test mumakonzo [Results] Migumisiro yeELISA neWestern blot yakaratidza kuti anti-KLH-PT1 uye anti-Agkistrodon acutus IgY inogona kuyambuka neAgkistrodon acutus uye Agkistrodon acutus venom. , uye yapashure yakanga yakasimba zvikuru kupfuura yokutanga;Anti-KLH-PT2 IgY haina muchinjiko wakanangana neAgkistrodon acutus venom uye Agkistrodon acutus venom in vitro, asi ine huturu hwakasimba husingadzoreki mukuedza kwekudzivirira kubuda ropa mu vivo, uye yakanyanya kukunda kune maviri ekare [Mhedziso] Iyo IgY. antibody inogona kuyambuka kuita neyakasiyana siyana yenyoka inotenderera neropa yakagadzirwa nekugadzira iyo yakajairika inoteedzana antigen peptide yemetalloproteinase, iyo yakateya nheyo yekugadzirira chaiyo, inoshanda, yakaderera muchetura uye yakafara-spectrum anti-hemorrhagic nyoka huturu hwekudzivirira.
Nguva yekutumira: Jan-12-2023