IgY antibody inopesana neyakajairika motif yemetalloproteinases kubva kuAgkistrodon acutus venom yakagadzirwa uye basa rayo mukuita kwehuturu hwenyoka rakadzidzwa [Nzira] Nekuongorora kutevedzana kwakajairwa kweakasiyana metalloproteinases kubva kuAgkistrodon acutus venom, isu takagadzira nekugadzirisa zvakanyanya kuchengetedzwa antigenic peptides PT1. , izvo zvine hukama zvakanyanya nemabasa avo ee enzyme;Huku dzakabayiwa ne conjugated complexes KLH-PT1 uye KLH-PT2 kuti dziwane IgY antibodies.Iyo titer yeIgY uye maitiro emuchinjikwa maitiro ane uturu hweAgkistrodon acutus uye Agkistrodon brevius zvakambodzidzwa neELISA uye Western blot;Pakupedzisira, kuita kweIgY kusingadzoreki kwakaongororwa neanti subcutaneous hemorrhage test mumakonzo [Results] ELISA uye Western blot yakaratidza kuti anti KLH-PT1 uye anti Agkistrodon acutus venom IgY inogona kuyambuka kuita neAgkistrodon acutus venom uye Agkistrodon brevius venom, uye huturu hwe latter. yakanga yakasimba zvikuru kupfuura yekare;Iyo anti KLH-PT2 IgY yakanga isina kuchinjika kwakakosha kuita neAgkistrodon acutus uye Agkistrodon brevius venoms in vitro, asi yakaratidza kusimba kwakasimba kwehupfu mukuedza kwekudzivirira kubuda ropa mu vivo, uye yaive yakanyanya kukunda kune maviri ekare [Mhedziso] Iyo IgY antibody inogona. react neropa rakasiyana siyana rinotenderera renyoka rakagadzirwa nekugadzira iyo antigen peptide yakagovaniswa nemetalloproteinases, iyo yakateya hwaro hwekugadzirira chaiyo, inoshanda, yakaderera muchetura uye yakafara-spectrum anti hemorrhagic nyoka uturu antibody.
Nguva yekutumira: Zvita-26-2022