Nyoka yehuturu hwe thrombin senge enzyme (TLE) is serine protease yemhuri yetrypsin, iyo ine yakawanda yakachengetedzwa kutevedzana netrypsin.Iyo ine arginine esterase chiitiko, inogona kuita zvakananga pafibrinogen, inokonzeresa kutsemuka kweArg2Gly peptide bond pane chaiyo chikamu chefibrinogen molecule, kuburitsa fibrinopeptide A (FPA) kana B (FPB. Mashoma alkaline thrombins akaparadzaniswa ne cation exchange column, yakasanganiswa ne gel filtration uye affinity chromatography.Affinity chromatography inoshandiswa zvakanyanya nekuda kwekunyanya kupatsanurwa kwayo uye yakakwira sampuli kudzoreredza.Maitirwo ekuchenesa akasiyana anoshandiswa, uye zvigadzirwa zvakawanikwa zvakasiyana.Guo Chunteng et al. [6, 7] vakawana thrombin sezvikamu P3 neP4 kubva muhuturu hweAgkistrodon acutus muFujian nekushandisa nzira dzakasiyana dzekunatsa.Zvidzidzo zvinoratidza kuti ese ane thrombin chiitiko, asi EDTA inovharisa zvishoma basa rethrombin yechikamu cheP4 uye inodzivirira zvachose coagulase kuita kwechikamu cheP3. Chikamu P4 hachina basa re activating factor
Nguva yekutumira: Zvita-23-2022