[Abstract] Chinangwa Kuona N-terminal 15 amino acid zvakasara zvezvikamu zviviri zvakafanana zveAgacutin.Iwo maviri ma subunits ee enzyme molecule anofanirwa kupatsanurwa kuti awane subunit imwe peptide cheni uye inoteedzana.Nzira dzakanatswa zvakanyanya Agacutin yakawanikwa nenzira yebiochemical.Single subunit samples dzekuzivikanwa uye kutevedzana dzakagadzirirwa neSDS-PAGE electrophoresis kupatsanurwa, subunit adhesive strip kupora uye PVDF membrane electrotransfer tekinoroji.Iyo yekupedzisira yakasarudza gumi neshanu amino acid zvakasara paN-terminal ye subunit neEdman degradation nzira.Mhinduro Kutevedzana kwechikamu chikuru (16 kDa) yaive DCSSGWSSYEEHQYY, uye iyo yediki subunit (15 kDa) yaive DSGWSSYEGHEYYV.Mhedziso Mhedziso yekutevedzana kwakaratidza kuti Agacutin yaive nyowani yenyoka uturu thrombin senge enzyme nekutsvaga NCBI dhatabhesi.
Nguva yekutumira: Zvita-22-2022